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Silencing Frizzled 2 (Fz2) results in reduced expression of selected genes in the Wnt signaling pathway. (A–F) Show mean expression levels of Wnt, Fz2, Dvl, GSK3β, β-catenin, and TCF, respectively, in dsGFP- and dsFs2-treated Cyrtorhinus lividipennis at 2 days after emergence (DAE). Relative mRNA levels were determined as stated above and normalized with β-actin. Data points are from three independent biological replicates and represent mean ± SEM.
