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. 2020 Sep 16;11:579233. doi: 10.3389/fphys.2020.579233

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Copyright © 2020 Ge, Jiang, Zheng, Zhou, Wu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing Frizzled 2 (Fz2) inhibits the expression and activity of selected components in the target of rapamycin (TOR) signaling pathway. (A–C) Show the mean expression levels of Rheb, TOR, and S6K at 2 days after emergence (DAE), respectively, in dsGFP- and dsFs2-treated Cyrtorhinus lividipennis. Relative mRNA levels were determined by comparison with standard curves using each gene and normalized using β-actin. (D) Shows the phosphorylation of S6K in fat body isolated from dsGFP- and dsFz2-treated C. lividipennis at 2 DAE. Phosphorylation was detected by Western blot analysis using phospho-p70-Thr-389 S6K polyclonal antisera; the loading control (β-actin) was analyzed using anti-β-actin antisera. Expression data points are from three independent biological replicates and are presented as the mean ± SEM.