FIGURE 5.

Frizzled 2 (Fz2) silencing inhibits the expression and production of Vg in the Cyrtorhinus lividipennis fat body. (A,B) Show the mean Vg expression and Vg protein levels at 2 days after emergence (DAE) in dsGFP- and dsFz2-treated C. lividipennis as determined by quantitative real-time PCR (qPCR) and Western blot analysis, respectively. (C,D) Show Vg expression at 1 DAE and 2 DAE. (E,F) Show Vg protein production as measured by Western blot analysis with antibody against β-actin as a loading control at 1 DAE and 2 DAE after methorprene and acetone treatments with new emergence dsGFP- and dsFz2-treated female adult. The relative mRNA levels were determined by comparison with the standard curve of the gene concerned and normalized against the β-actin gene. β-actin antibody was used as the loading control. The relative gray values normalized to β-actin were marked above corresponding bands. Expression data are from three independent biological replicates and are presented as the mean ± SEM.