FIGURE 9.

Effects of dsFz2 treatment on eggs and embryonic development at 2, 4, and 6 days after emergence (DAE). (A–C) Show the phenotype of eggs produced by mated Cyrtorhinus lividipennis females treated with dsGFP at 2, 4, and 6 DAE, respectively. (D–F) Show eggs laid by mated C. lividipennis injected with dsFz2 at 2, 4, and 6 DAE, respectively. In (G–I), the nuclear stain 4’,6-diamidino-2-phenylindole (DAPI) was used to stain eggs in the dsGFP-treated females at 2, 4, and 6 DAE, respectively. (J–L) Show eggs of dsFz2-treated females stained with DAPI at 2, 4, and 6 DAE, respectively. Fluorescence images were acquired using a Zeiss LSM 780 confocal microscope. Red ovals and arrows indicate the location of eyespots. Scale bar, 100 or 200 μm.