Table 4.
Target gene, primer sequence (forward and reverse), and accession number for all gene transcripts analyzed by real-time PCR
| Target gene | Primer sequence | Accession no. |
|---|---|---|
| Peroxisome proliferator-activated receptor-γ | NM_181024.2 | |
| Forward | GTGAAGTTCAACGCACTGGA | |
| Reverse | ATGTCCTCAATGGGCTTCAC | |
| Sterol regulatory element-binding protein-1 | NM_001113302.1 | |
| Forward | ACCGCTCTTCCATCAATGAC | |
| Reverse | GCTGAAGGAAGCGGATGTAG | |
| Stearoyl-CoA dessaturase-1 | NM_173959.4 | |
| Forward | GCCAGAGTTGGGTCAAACAT | |
| Reverse | CTCTTCTTCAAGCCCCCTCT | |
| Lipoprotein lipase | NM_001075120.1 | |
| Forward | CTCCTGATGATGCGGATTTT | |
| Reverse | ACATCCTGGTTGGAAAGTGC | |
| Beta-actin | BC142413.1 | |
| Forward | GATGAGATTGGCATGGCTTT | |
| Reverse | GTCACCTTCACCGTTCCAGT | |
| Glyceraldehyde-3-phosphate dehydrogenase | NM_001034034.1 | |
| Forward | TGACCCCTTCATTGACCTTC | |
| Reverse | GATCTCGCTCCTGGAAGATG |