Table 1:

Kinetic Constants for Correct and Incorrect Nucleotide Incorporation^a

dNTP	Kd,1 (μM)	k2 (s−1)	k−2 (s−1)	k3 (s−1)	kcat/Km (μM−1 s−1)	kcat (s−1)	Km (μM)
dCTP	28	660	1.6	360	22	230	10
dGTP	200	220	420	0.3	0.0008	0.1	130
	$\mathrm{E}\cdot {\mathrm{D}}_n+\mathrm{N}\underset{k_{-1}}{\overset{k_1}{\rightleftarrows }}\mathrm{E}\cdot {\mathrm{D}}_n\cdot \mathrm{N}\underset{k_{-2}}{\overset{k_2}{\rightleftarrows }}\mathrm{F}\cdot {\mathrm{D}}_n\cdot \mathrm{N}\stackrel{k_3}{\to }\mathrm{E}\cdot {\mathrm{D}}_{n+1}+{\text{PP}}_i$

^aKinetic constants for correct (dCTP) and incorrect (dGTP) nucleotide binding and incorporation are listed and defined according to the model shown in the table. Kinetic parameters k_cat, k_cat/K_m, and K_m were calculated from the individual rate constants listed.