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. Author manuscript; available in PMC: 2020 Sep 30.
Published in final edited form as: Biochemistry. 2012 Mar 22;51(13):2852–2866. doi: 10.1021/bi201479k

Figure 4.

Figure 4.

Effect of the single-amino acid substitutions on cis-(Z)-flupentixol-stimulated ATP hydrolysis. The vanadate-sensitive ATP hydrolysis by the wild type and alanine-substituted Pgps was measured in the presence of varying concentrations (0–100 μM) of Pgp modulator cis-(Z)-flupentixol. For each TM helix, the cis-(Z)-flupentixol-stimulated ATPase activity of Pgp alanine mutants were compared to that of wild-type Pgp. The data were expressed as fold stimulation of the basal activity as a function of cis-(Z)-flupentixol concentration. Each point represents the mean value of the data obtained from three independent experiments. Data were analyzed by nonlinear regression using GraphPad PRISM 4.