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. Author manuscript; available in PMC: 2020 Sep 30.
Published in final edited form as: Biochemistry. 2012 Mar 22;51(13):2852–2866. doi: 10.1021/bi201479k

Figure 8.

Figure 8.

Negative synergism between the stimulatory effects of ds-(Z)-flupentixol and verapamil on ATP hydrolysis. ATP hydrolysis by the wild type and Pgp mutants I864A, Q990A, and I765A, in isolated membrane vesicles, was conducted either after preincubation with 50 ^M verapamil (top) or with 50 ^M ds-(Z)-flupentixol (bottom) followed by incubation with increasing concentrations of ds-(Z)-flupentixol (top) or verapamil (bottom), using the vanadate-sensitive phosphate release assay. Data are expressed as fold stimulation of the basal ATPase activity observed for respective Pgp mutants or the wild type. Each set represents averages of three independent experiments.