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. 2020 Apr 21;29(9):1498–1519. doi: 10.1093/hmg/ddaa070

Figure 1.

Figure 1

GTF2IRD1 binds preferentially to promoters in conserved, active sites in the genome. (A) GTF2IRD1 and GTF2I IP experiment, followed by immunoblotting for both proteins, shows that each antibody is specific for each protein. (B) GTF2IRD1 binding peaks are annotated primarily in promoters and gene bodies. The distribution of peak annotations is significantly different from random sampling of the genome. (C) GTF2IRD1 peaks were enriched in H3K4me3 sites marking active regions of the genome and to a lesser extent in H3K27me3 sites marking repressed regions. (D) GO analysis of genes that have GTF2IRD1 bound to the promoter. (E) The conservation of sequence in GTF2IRD1-bound peaks is significantly higher than random regions of the genome and a random set of promoter regions. (F) Motifs of transcription factors enriched under GTF2IRD1-bound peaks. *p < 0.05, **p < 0.01, ***p < 0.001.