TABLE 6.
Mass spectrometry analyses of BlaMab, LdtMab1 to LdtMab5, and d,d-carboxypeptidase alone and incubated with imipenem, ceftaroline fosamil, imipenem plus ceftaroline fosamil, relebactam, and avibactam
| Protein (nominal mol wt) | Compound (nominal mol wt) | Observed mol wta (±5) | Change in mol wta |
|---|---|---|---|
| BlaMab (28,433) | None | 28,433 | |
| Avibactam (265) | 28,698 | +265 | |
| Relebactam (349) | 28,782 | +349 | |
| Ceftaroline fosamil (685) | 28,434 | +0 | |
| Imipenemb (299) | 28,728 | +299 | |
| LdtMab1 (23,059) | None | 23,059 | |
| Avibactam (265) | 23,325 | +265 | |
| Relebactam (349) | 23,407 | +348 | |
| Ceftaroline fosamil (685) | 23,744 23,664 | +685 +605 | |
| Imipenem (299) | 23,359 23,316 (minor) | +300 +257 | |
| Imipenem (299) Ceftaroline fosamil (685) | 23,359 23,664 (minor) | +300 +605 | |
| LdtMab2 (39,216) | None | 39,216 | |
| Avibactam (265) | 39,482 | +266 | |
| Relebactam (349) | 39,565 | +349 | |
| Ceftaroline fosamil (685) | 39,901 39,821 (minor) | +685 +605 | |
| Imipenem (299) | 39,515 | +299 | |
| Imipenem (299) Ceftaroline fosamil (685) | 39,516 | +300 | |
| LdtMab3 (44,795) | None | 44,795 | |
| Avibactam (265) | 44,794 | +0 | |
| Relebactam (349) | 44,795 | +0 | |
| Ceftaroline fosamil (685) | 44,795 | +0 | |
| Imipenem (299) | 44,795 | +0 | |
| Imipenem (299) Ceftaroline fosamil (685) | 44,795 | +0 | |
| LdtMab4 (32,567) | None | 32,567 | |
| Avibactam (265) | 32,566 32,831 (minor) | +0 +265 | |
| Relebactam (349) | 32,567 | +0 | |
| Ceftaroline fosamil (685) | 33,252 33,171 (minor) | +685 +604 | |
| Imipenem (299) | 32,866 32,824 (minor) | +299 +257 | |
| Imipenem (299) Ceftaroline fosamil (685) | 32,865 32,821 (minor) | +298 +254 | |
| LdtMab5(H6) (28,220) | None | 28,220 | |
| Avibactam (265) | 28,220 | +0 | |
| Relebactam (349) | 28,220 | +0 | |
| Ceftaroline fosamil (685) | 28,220 28,825 28,905 | +0 +605 +685 | |
| Imipenem (299) | 28,476 28,520 | +256 +300 | |
| Imipenem (299) Ceftaroline fosamil (685) | 28,476 28,519 | +256 +299 | |
| M. abscessus d,d-carboxypeptidase (26,791) | None | 26,791 | |
| Avibactam (265) | 26,791 27,057 | +0 +265 | |
| Relebactam (349) | 26,791 | +0 | |
| Ceftaroline fosamil (685) | 26,790 (minor) 27,396 | +0 +605 | |
| Imipenem (299) | 27,046 (minor) 27,090 | +255 +299 | |
| Imipenem (299) Ceftaroline fosamil (685) | 27,090 27,046 (minor) | +299 +255 |
a
Mass accuracy, ±5 Da.
b
Complex captured at 15 s.