FIG 3.
Survival curves of Galleria mellonella following infection with Candida auris. G. mellonella larvae were infected with 2.5 × 105 cells/larvae of C. auris ± chitosan treatment, and survival rates were monitored every 24 h for 4 days. A total of 3 chitosan treatments were used (50 mg/kg, green line; 100 mg/kg, yellow line; and 200 mg/kg, blue line). Control groups received PBS only (black line) or were infected with C. auris minus chitosan treatment (red line). The highest concentration of chitosan had no effect on the survival of the larvae minus C. auris infection (data not shown). Data representative of results from three independent experiments with 10 larvae per group are shown in a Kaplan-Meier plot, and statistical differences are calculated between treatment groups by the log rank (Mantel-Cox) test. *, Significant differences between highest concentration of chitosan (200 mg/kg) and infected controls minus chitosan treatment (*, P < 0.05; ****, P < 0.0001).