FIG 4.
Fungal load from Candida auris-infected Galleria mellonella. G. mellonella larvae were infected with 2.5 × 105 cells/larvae of C. auris ± chitosan treatment. After 24 h, larvae were harvested and weighed prior to DNA extraction. The abundance of C. auris (presented as colony forming equivalents per gram) in the larvae was calculated by quantitative PCR using a standard curve methodology of fungal CFU ranging from 1 × 103 to 1 × 108 CFU/ml. Significant differences were calculated using a one-way analysis of variance (ANOVA) with Tukey’s posttest. Significant differences denoted by an asterisk (*, P < 0.05). Data representative of results from three independent experiments.