FIG 5.

Gene expression profile of Candida auris in the Galleria mellonella infection model. G. mellonella larvae were infected with 2.5 × 10⁵ cells/larvae of C. auris ± three concentrations of chitosan treatment (50 mg/kg, 100 mg/kg, and 200 mg/kg). After 24 h, larvae were harvested for RNA extraction. Gene expression was measured by quantitative PCR, and expression of all genes of interest was calculated relative to a housekeeping gene (β-actin). Data were presented as mean values from three independent experiments in a heatmap, calculated as log₂ fold change of expression in C. auris NCPF 8973 relative to C. auris NCPF 8978 ± chitosan treatment.