TABLE 2.
List of primer sequences used in this studya
| Study | Gene | Direction | Sequence (5′–3′) |
|---|---|---|---|
| Fungal load quantification | ITS | Forward | TCGCATCGATGAAGAACGCAGC |
| Reverse | TCTTTTCCTCCGCTTATTGATATGC | ||
| Gene expression studies | β-actin | Forward | GGCTCATCTTGGCTTCCTT |
| Reverse | GGACCGGACTCGTCGTATTC | ||
| SAP5 | Forward | GGATGCAGCTCTTCCTGGTT | |
| Reverse | CTTCCAGTTTGCGGTTGTGG | ||
| PLB1 | Forward | TGCCATCTACAACCCGAACC | |
| Reverse | TCAACGACGACAAGGGAAGG | ||
| ENG1 | Forward | TGTGAAGGATGAGGCTGCTG | |
| Reverse | GTGCTAGTCACACCACCGAA | ||
| ERG2 | Forward | ACACAAAGCCGAATGGCAAC | |
| Reverse | GAGAGGCCAAGTGAAGCAGT | ||
| ALS5 | Forward | ATACCAGGGTCGGTAGCAGT | |
| Reverse | CTATCTTCGCCGCTTGGGAT | ||
| HYR3 | Forward | TTCGACTTCCCTGAGCCAAC | |
| Reverse | AGCTCGAAACAGCAGACGAA | ||
| KRE6 | Forward | ATCACGATCGACATGGGCTC | |
| Reverse | TCAACGACAACGAAAACGGC | ||
| EXG | Forward | CAACAAAGGCGTCAACTGGG | |
| Reverse | TTCATCCACAGGGACAGTGC |
a
Forward and reverse primer sequences for C. auris fungal load quantification and gene expression analyses in this study.