Fig 3. N-WASP is partially required for recruitment of the ARP2/3 complex to the EB invasion site.

N-WASP knockdown cells were infected at a MOI of 5 for 30min and stained using anti-ARP2/3 (red) and anti-LPS (green) antibodies. Images were captured by confocal microscopy. The * denotes area used for orthogonal view. Scale bar is 20μm. Recruitment was assessed from 30 fields of view per experiment. Knock down efficiency was confirmed by western blotting. Data are representative of 2 independent experiments. Statistical significance was determined using t-test. ** P<0.01