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. 2020 Sep 18;16(9):e1008878. doi: 10.1371/journal.ppat.1008878

Fig 7. The secreted effector proteins TmeA and TarP target distinct signaling pathways that converge on the ARP2/3 complex.

Fig 7

(A) Human cervical cells were infected at a MOI of 5 for 60min with wild-type, tmeA-lx, tarP::bla, or tmeA-lx tarP::bla. The number of internal bacteria were enumerated from 30 fields of view per experiment using differential immunostaining. Scale bar is 20μm. (B) Human cervical cells were infected at a MOI of 5 for 30min with wild-type, tmeA-lx, tarP::bla, or tmeA-lx tarP::bla and chlamydial EBs were stained using an anti-LPS antibody (green) and anti-N-WASP or anti-ARP2/3 (red) antibodies were used to visualize host factors. Recruitment was assessed from 30 fields of view per experiment. Scale bar is 0.3μm. (C) N-WASP and ARP3 knock down cervical cells were infected at a MOI of 5 for 60min with wild-type, tmeA-lx, tarP::bla, or tmeA-lx tarP::bla. The number of internal bacteria was determine using differential immunostaining. (A-C) Data are representative of 2 independent experiments. Statistical significance was determined using One-Way ANOVA. *** P<0.001, **P<0.01, *P<0.05.