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. 2020 Sep 30;15(9):e0239428. doi: 10.1371/journal.pone.0239428

Fig 8. Proliferation of mitochondria by the treatment of acetic acid or GPR43 agonist through the activation of GPR43.

Fig 8

(A) Genomic DNA was extracted from L6 cells that were transfected or non-transfected with GPR43- siRNA after the treatment or no treatment of 0.5 mM acetic acid or 1.0 μM GPR43 agonist for 30 min. Real-time PCR analysis was carried out for the determination of mt-Nd1 level in L6 myotube cells. (B) SDH activity (nmol/min/mg of protein) in L6 cells of same condition with (A) was measured by consumption rate of DCPIP as described in Materials and Methods. Each bar represents the mean ± SE (n = 3–6). Multiple comparisons were analyzed with one-way ANOVA followed by the Tukey-Kramer post hoc test. Statistical differences are shown as *p< 0.05, **p< 0.01, compared with non-treated control; #p< 0.05, ##p< 0.01, compared with GPR43 siRNA; §p< 0.05, §§p< 0.01, compared with GPR43 siRNA + ace; op< 0.05, oop< 0.01, compared with GPR43 siRNA + agonist.