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. 2020 Sep 29;94(20):e01146-20. doi: 10.1128/JVI.01146-20

FIG 1.

FIG 1

Purification of GCN4-CT38. (A) Electrophoreogram illustrating the purification of GCN4-CT38. (B) Chromatogram illustrating the separation of the protein standard mix (Sigma-Aldrich; red) and the GCN4-CT38 sample (the same as in lane S3 panel A; blue). PL, Thermo Scientific PageRuler Plus prestained protein ladder; P1 and S1, the pellet and supernatant, respectively, of the centrifuged lysate of E. coli BL21(DE3) producing GCN4-CT38; P2 and S2, the pellet and supernatant, respectively, obtained after the precipitation by 33% ammonium sulfate; P3 and S3, the pellet and supernatant, respectively, obtained after the overnight resuspension of GCN4-CT precipitated in P2; F, the final GCN4-CT purified from S3 by size exclusion chromatography and concentrated using microfiltration.