FIG 5.

Linkages identified between the MA and GCN4-CT38 by XL-MS. The schemes of the proteins are displayed in violet (GCN4-CT38) or gray (MA) cylinders. The GCN4 part of the GCN4-CT38 is colored a dark shade of violet, and the CT part is a light shade of violet. The MA, PP, and His tag parts of MAPPHis are colored different shades of gray. The linkages identified in three repetitions of the analysis are depicted as lines between cylinders. The linearized structure of the MA is presented under the MA with schematically displayed helices in the MA structure. The sequences of identified cross-linked peptides are shown in the rectangle on the right, where the sequences and residues of GCN4-CT38 are colored violet and the sequences and residues of the MA are colored gray. The obtained spectra of the cross-links K34/T37-K16 and K34/T37-K27 did not allow determination of whether the residues K16 and K27 of MAPPHis interacted with the residue K34 or T37 of GCN4-CT38.