TABLE 1.
Cross-links between GCN4-CT38 and MAPPHis identified by XL-MSa
| m/z measured | Calculated mol wt (Da) | Charge state | Mass deviation (ppm) | GCN4-CT38 peptide | MAPPHis peptide | Linked residues (GCN4-CT38 to MAPPHis) |
|---|---|---|---|---|---|---|
| 561.589 | 2,243.337 | 4 | −0.67 | KLIGEANK | GVKVKYADLLK | K27-K27 |
| 709.409 | 2,834.610 | 4 | 1.90 | LIGEANKLMTFIK | YVEQLKQALK | K34/T37-K16 |
| 712.924 | 2,848.662 | 4 | 4.36 | LIGEANKLMTFIK | GVKVKYADLLK | K34/T37-K27 |
| 715.097 | 2,143.275 | 3 | 0.63 | LMTFIK | GVKVKYADLLK | T37-K27 |
| 620.367 | 1,859.090 | 3 | −1.43 | LMTFIK | VKYADLLK | T37-K27 |
a
For all the cross-links, the table shows the measured m/z value, the calculated molecular weight in daltons, the charge state, mass deviation, GCN4-CT38 and MAPPHis peptides, and their residues linked by BS3. The linked residues are marked in bold in the peptide sequences.