FIG 3.

HMPV inclusion body formation in HAE. (A) HAE tissues were infected with rgHMPV and stained for the nucleoprotein, N, and phosphoprotein, P, to determine the formation of inclusion bodies in HAE tissues. (B) Colocalization of N and P was analyzed using colocalization chromatography from NIS-Elements. (C) To confirm inclusion body formation in HAE tissues, FISH analysis was conducted to label vRNA. (D) Both vRNA and HMPV N colocalize to cytosolic punctate structures in infected cells. (E) vRNA was also assessed in relation to HMPV using fluorescence microscopy. (F) Chromatogram analysis demonstrates that P and vRNA colocalize with one another in infected cells. Scale bar = 10 μm for combined images with DIC and 5 μm for fluorescence insets.