FIG 8.

54G10 significantly inhibits spread of HMPV. (A) HAE tissues were infected with rgHMPV at MOI of 3.0. Tissues were treated apically with 54G10 6 hours postinoculation in 50 μl of TEER buffer. Fluorescence microscopy images were taken up to 72 hours postinfection. (B) Tissues were fixed at 48 hpi and microtome-sectioned for each treatment group and examined for viral spread. (C) Fluorescence threshold analyses of inverted fluorescence microscopy images demonstrate that 54G10 modestly but significantly prevents the spread of rgHMPV in HAE infected tissues. Scale bar = 10 μm. Error bars represent SEM of 6 tissues per treatment group. Statistical significance is represented with P < 0.05 (*), P < 0.005 (**), P < 0.0005 (***), and P < 0.0001 (****).