FIG 11.

Effect of anti-gH/gL Fabs on gH2/gL2 binding to gD2. Experiments were set up as outlined in Fig. 9A. Anti-gD MAb 1D3 was coupled to a CM5 biosensor chip and then soluble gD2(285t) was injected across the flow cell. Next, either gH2/gL2 alone or gH2/gL2 that was preincubated with 0.6 mg/ml of the indicated Fab was injected across the flow cell. Fab concentration was chosen so it was in excess. The RU from a control flow cell where only gH/gL or gH/gL + Fab was injected (no gD) was subtracted from each. Only gH/gL binding curves are shown. Black curves show gH/gL binding to gD when no Fab is added (positive control). Curves where gH/gL was preincubated with Fabs are colored according to their MAb community in Fig. 2B.