FIG 4.

Positioning of MAb epitope communities onto a 3D structural model of the full-length gH2/gL2 ectodomain. Surface representation of gH2 is shown in white while gL2 is shown in gray. The structure is rotated 180^° between (A) and (B). Antibody-resistant mutations for individual MAbs and peptide mapping data (28, 31, 33) were used to position the colored MAb communities (red, magenta, orange, cyan, yellow, and purple) from Fig. 1C onto the structural model. These residues are similarly colored on the surface of the gH2/gL2 structure to convey points of orientation. Due to the large number of MAbs within the red community, only CHL2 is shown. MAbs CHL21 and CHL37, which strongly compete with type 1 MAbs 52S and BBH5, are positioned above the MAb-resistant mutations for 52S (gH 536) (33) and BBH5 (gH 732), shown on the structure in purple. MAb CHL27 was mapped to gH residues 37 to 47 and MAb 53S to gL residue 77, as shown in subsequent figures.