FIG 5.

Epitope mapping: characterization of MAbs CHL21 and CHL37. (A) Western blot analysis. Soluble gH/gL proteins were run under either native (N) or denaturing (D) conditions. Blots were probed with the indicated antibodies. R137 was used as the PAb against gH1/gL1, and R176 as the PAb against gH2/gL2 and gH2Δ48/gL2. Molecular weight markers are designated with lines and shown to the left in kDa. (B) SPR analysis of gH/gL binding. Anti-HIS IgG was coupled to a biosensor chip. Next, soluble, purified gH1/gL1 (gray), gH2/gL2 (black), or gH2Δ48/gL2 (brown) was captured via C-terminal 6-HIS tags. Curves show binding of the indicated MAb to the immobilized proteins. Each experiment was done at least twice and a representative experiment shown.