FIG 6.

Effect of M25 proteins on p53 association with promoter sequences. NIH 3T3 were either used uninfected or were infected with WT MCMV or the ΔM25 mutant (MOI of 3) or were treated with doxorubicin (Doxr) for 18 h. Chromatin immunoprecipitation (ChIP) was performed using a p53 antibody or IgG for control, and the amounts of precipitated and input DNA were quantified by qPCR. The values represent the percentage of precipitated DNA (means plus the SD of four biological replicates). A t test (one-tailed) was used for statistical analysis.