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. 2020 Sep 29;94(20):e01110-20. doi: 10.1128/JVI.01110-20

FIG 1.

FIG 1

HPV E2 interacts with Pyk2. (A) HEK293TT cells were transfected with GFP-Pyk2 WT (wild type) or GFP-Pyk2 KD (kinase dead) and FLAG-HPV-31 E2. FLAG-HPV-31 E2 was immunoprecipitated with M2 (FLAG) antibodies. Complexes were blotted with M2 (FLAG) and Pyk2 antibodies. (B) HEK293TT cells were transfected with GFP-Pyk2 WT and FLAG-HPV-16 E2. FLAG-HPV-16 E2 was immunoprecipitated with M2 (FLAG) antibodies. Complexes were blotted with M2 (FLAG) and Pyk2 antibodies. (C) HPV-16 E2 in W12 cells was immunoprecipitated using sheep anti-HPV-16 E2 serum or normal sheep IgG. Complexes were blotted with Pyk2 and HPV-16 E2 (TVG-261) antibodies.