Skip to main content
. 2020 Sep 29;94(20):e01110-20. doi: 10.1128/JVI.01110-20

FIG 10.

FIG 10

Y131E is unable to stimulate transient replication and is defective for Brd4 CTM binding. (A) C33A cells were transfected with HPV-31 E1 and HPV-31 Y131 mutants, along with pFLORI31 and pRL constructs. After 72 h later, the firefly and Renilla luciferase levels were measured using Dual-Glo luciferase reagent. The firefly luciferase levels were normalized to the Renilla luciferase levels. Values are expressed as means ± the SEM (n = 8). (B) HEK293TT cells transfected with HA-HPV-31 E1 and FLAG-HPV-31 E2 Y131 mutants. HPV-31 E2 was immunoprecipitated with M2 (FLAG) antibodies. Complexes were blotted with E1 and M2 (FLAG) antibodies. (C) HEK293TT cells were transfected with FLAG-ChlR1 and FLAG-HPV-31 E2 Y131 mutants. Immunoprecipitations were performed with goat ChlR1 antibodies and immunoblotted with M2 (FLAG) antibodies. (D) HEK293TT cells transfected with FLAG-Brd4 full-length and FLAG-HPV-31 E2 Y131 mutants. Immunoprecipitations were performed with Brd4 antibodies and immunoblotted with M2 (FLAG) antibodies. (E) HEK293TT cells transfected with GST-CTM and FLAG-HPV-31 E2 Y131 mutants. HPV-31 E2 was immunoprecipitated with M2 (FLAG) antibodies. Complexes were blotted with GST and M2 antibodies.