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. 2020 Sep 29;94(20):e01110-20. doi: 10.1128/JVI.01110-20

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FIG 2 — Pyk2 interacts with the TAD of E2. (A) Illustration of HPV-31/-16 E2 fragments. (B) HEK293TT cells were transfected with GFP-Pyk2 and FLAG-HPV-31 E2 or HPV-31 E2 fragments (aa 107 to 372, 176 to 372, and 205 to 372) and immunoprecipitated with M2 (FLAG) antibodies. Complexes were blotted with M2 (FLAG) and Pyk2 antibodies. (C) HEK293TT cells were transfected with GFP-Pyk2 and FLAG-HPV-31 E2 or HPV-31 E2 with the β-sheet region of the E2 TAD deleted (aa 1 to 106 and aa 176 to 372) and immunoprecipitated with M2 (FLAG) antibodies. Complexes were blotted with M2 (FLAG) and Pyk2 antibodies. (D) HEK293TT cells were transfected with GFP-Pyk2 and FLAG-HPV-16 E2 or HPV-16 E2 fragments (aa 1 to 104, 202 to 365, 105 to 365, and 202 to 365). After immunoprecipitation with M2 (FLAG) antibodies, complexes were blotted with M2 (FLAG) and Pyk2 antibodies.