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Pyk2 induced tyrosine phosphorylation of HPV-31 E2 Y to F mutants. HEK293TT cells were transfected with GFP-Pyk2 and FLAG-HPV-31 E2 phenylalanine F mutants (A) or with GFP-Pyk2 WT or GFP-Pyk2 KD and FLAG-HPV-31 F mutants (B). Lysates were immunoprecipitated with M2 (FLAG), and bound proteins were blotted with Pyk2, pTyr-1000, and M2 (FLAG) antibodies. (C) Quantification of phosphotyrosine levels of Y131F. Values are means ± the SEM (n = 3). *, P < 0.05 (two-way t test).
