FIG 6.

Pyk2 siRNA increases HPV DNA content. (A) CIN612-9E cells were transfected with a scrambled control, Pyk2, or EGFR siRNA. After 72 h, lysates were immunoblotted with Pyk2, EGFR, or β-actin antibodies. CIN612-9E cells were transfected with a control, Pyk2, or EGFR siRNA. Real-time PCR was performed for the HPV-31 long control region (LCR) and normalized to the level of β-actin. Values are expressed as means ± the SEM (n = 6). *, P < 0.05. (B) W12 cells were transfected with a control, Pyk2, or EGFR siRNA. After 72 h, the lysates were immunoblotted with Pyk2, EGFR, and β-actin antibodies. W12 cells were transfected with a control, Pyk2, or EGFR siRNA. DNA was isolated for real-time PCR for the HPV16 E6 DNA region and normalized to the levels of β-actin. Values are expressed as means ± the SEM (n = 12). *, P < 0.05.