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. 2020 Sep 29;94(20):e01110-20. doi: 10.1128/JVI.01110-20

FIG 8.

FIG 8

Pyk2 knockout increases HPV genome integration. (A) CIN612-9E cells infected with pLentiCRISPR v2 (control) or pLentiCRISPR v2-Pyk2 guide constructs. Lysates were immunoblotted with Pyk2 and β-actin antibodies. (B) DNA from control and Pyk2 knockout (KO) CIN612-9E cell lines were analyzed by qPCR for the HPV-31 LCR and normalized to β-actin. Values are expressed as means ± the SEM (n = 9). (C) DNA from control and Pyk2 KO CIN612-9E cell lines were subjected to exonuclease V digestion. Resistant HPV-31 DNA was quantified by qPCR. Actin DNA was used as a positive control for digestion, and mitochondrial DNA was used as a resistant control. Values are expressed as means ± the SEM (n = 9) *, P < 0.05. (D). Real-time PCR was performed using primers for HPV-31 E1̂E4, E8̂E2, and E6 transcripts and normalized to actin transcripts. Values are means ± the SEM (n = 3). *, P < 0.05.