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. 2020 Sep 29;94(20):e01110-20. doi: 10.1128/JVI.01110-20

FIG 9.

FIG 9

E2 Y131 is important for episomal maintenance. (A) NIKS cells were infected with HPV-31 WT and mutant quasiviruses and selected with G418 for 2 weeks. Quantification of viral HPV-31 DNA was carried out using qPCR in NIKS cells infected with HPV-31 WT and Y131F quasiviruses. Values are expressed as means ± the SEM (n = 3). (B) CIN612 episomal and integrated cell lines served as controls in the exonuclease V digestion assay. HPV-31 DNA from NIKS WT and Y131F quasiviruses was quantified using real-time PCR after exonuclease V digestion for actin and mitochondrial DNA. Values are means of the percent exonuclease V resistance ± the SEM (n = 3). (C) Percent exonuclease V resistance for HPV-31 DNA in CIN612 and NIKS WT and Y131F cell lines. Values are means ± the SEM (n = 3) *, P < 0.05 (compared to CIN612 cells by one-way t test). (D) NIKS cells were infected with HPV-31 WT and mutant quasiviruses and selected with G418 for 28 days. Quantification of HPV-31 DNA using qPCR in NIKS cells infected with HPV-31 WT and Y131 mutant quasiviruses. Values expressed as means ± the SEM (n = 3).