FIG 1.

High clonal overlaps between the stem-like and exhausted subsets are observed within an individual mouse. (a) Serum viral load during chronic LCMV infection. (b) Mice were sacrificed at days 45 to 60 p.i. to isolate LCMV-specific stem-like and/or exhausted CD8 T cells from the spleen and liver for TCRβ sequencing. (c) Antigen-specific stem-like and exhausted CD8 T cells were sorted using pMHC tetramers, Tim3, and CD73. (d and e) The Morisita-Horn index was used to quantify the clonal overlap between the GP33-specific and GP276-specific stem-like (Stem) and exhausted (Exh) subsets within an individual mouse. Shown are the mean and standard deviation (SD) from experiments with 4 mice.