FIG 3.

The TCR repertoire of the stem-like subset is more broad and diverse compared to the exhausted. (a and b) GP33⁺ (n = 4) (a) and GP276⁺ (n = 4) (b) frequency distribution of each clonotype within individual mice. Each color indicates the same TCR clonotype only within an individual mouse. (c) Number of clonotypes that make up 95% of the repertoire in each of the subsets. (d) Cumulative frequency of clonotypes that are shared among all subsets in an individual mouse. (e) Cumulative frequency of the 5 most dominant clonotypes in each subset. (f) Quantification of diversity using Simpson’s D where D = 1 is monoclonal and D = 0 is completely polyclonal. Shown are the mean and SD from experiments with 4 mice. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001 (all determined using paired analysis of variance [ANOVA] with multiple comparisons).