TABLE 2.
Cryo-EM data collection and refinement
| Parameter | Value or description |
|---|---|
| Data collection | |
| Magnification | ×75,000 |
| Voltage (kV) | 300 |
| Electron dose (e−/Å−2) | 30 |
| Defocus angle (μm) | −0.5 to −1.5 |
| Pixel size (Å) | 1.053 |
| Map reconstruction | |
| No. of particles overall | 23,349 |
| No. of particles used | 22,122 |
| Resolution (Å) (FSC = 0.143) | 3.42 |
| Map-sharpening B-factor (Å2) | −90 |
| Real-space refinement | |
| Software (version) | PHENIX (1.14) |
| Model resolution (Å) (FSC = 0.143) | 4.13 |
| No. of atoms | 969,900 |
| No. of protein residues | 110,701 |
| Map-to-model CC | |
| CCmask | 0.82 |
| CCvolume | 0.82 |
| CCpeaks | 0.74 |
| CCbox | 0.78 |
| Bond RMSD (Å) | 0.007 |
| Angle RMSD (°) | 1.14 |
| Ramachandran plot (%) (favored/allowed/outliers) | 93.14/6.81/0.0 |
| Rotamer outliers (%) | 1.91 |
| All-atom clash score | 8.07 |
| Avg B-factor (Å2) | 105.92 |