PRVABC59 L330 produces low viral loads late in infection. Five- to six-week-old Ifnar1−/− mice were inoculated with 1 × 103 FFU of ZIKV strain PRVABC59 V330 or L330 virus by a subcutaneous route in the footpad. (A) Blood was collected 2, 4, and 6 days after infection, and ZIKV RNA in serum was measured by qRT-PCR. (B to E) Mice were euthanized 6 days after infection and perfused, and tissues were harvested. ZIKV RNA in tissue was measured by qRT-PCR. (F) V330-infected mice were euthanized when they reached a humane endpoint (8 or 9 dpi) and L330-infected mice were euthanized at 9 dpi, tissues were harvested, and ZIKV RNA in the brain was measured by qRT-PCR. ****, P < 0.0001 (unpaired 2-tailed t test). Results shown represent data from 2 independent experiments.