TABLE 2.
Comparison of sterol profiles between B. minutum and F. kawagutiia
| Sterol | Structure | B. minutum (%) | F. kawagutii (%) |
|---|---|---|---|
| 24-Nor-23-demethylgorgosterol | B1 (H1) b , c | 0.9 | —d |
| Cholesterol | B2 (H2) c | 10.6 | — |
| 23,24-Demethylgorgosterol | B3 c | 0.4 | — |
| Brassicasterol | B4 (H4) c | 2.8 | — |
| 4-Methylcholest-22-enol | F1 | — | 6.9 |
| Lophenol | B5 (F2) | 0.5 | 7.5 |
| Lophanol | F3 | — | 5.3 |
| 22-Dihydrobrassicasterol | B6 | 5.4 | — |
| 4,23-Dimethylcholest-7-enol | F4 | — | 13.3 |
| 24-Demethylgorgosterol | B7 c | 1.9 | — |
| 24-Demethyldinosterol | B8 (F5) | 27.2 | 18.4 |
| 4,24-Dimethylcholesta-7,22-dienol | B9 | 0.5 | — |
| 4,24-Dimethylcholest-7-enol | B10 (H15) | 0.9 | — |
| 23,24-Demethylcholesta-5,22-dienol | B11 c | 0.6 | — |
| 4,24-Dimenthylcholestanol | B12 (F6, H16) | 18.8 | 8.3 |
| Dinosterol | B13 (F7, H17) | 12.4 | 40.4 |
| Gorgosterol | B14 c | 17.0 | — |
a
Cultures in exponential phase (1 × 106 cells·ml−1) were collected for sterol profiling. Three biological replicates of algal cultures were established.
b
The structure numbers in boldface and their corresponding mass spectra are listed in Fig. S2b and c. “H,” “B,” and “F” indicate sterols identified in the anemone H. crispa, B. minutum, and F. kawagutii, respectively. For molecules identified in two or more species, their designations in other biological samples are shown in parentheses.
c
Δ5 sterol.
d
Dashes (—) indicate values below the detection limit.