Fig. 5. Efficient screening of the cryptic site–bound peptide by the F143W mutant.

(A) Comparison of the enrichment factors in each round of phage-display screening. The ratio of hit clone against the whole clone cfu was shown for each panning round. (B) Normalized CSP induced by the hit peptide; 160 μM peptide was added to 100 μM WT Bcl-xL. (C) Mapping of the residue that experienced normalized CSP > 0.01 ppm. (D) Correlation plot of the ABT-737–induced allosteric ¹³C CSPs against the peptide-induced ¹³C CSPs.