Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 30;9:e59754. doi: 10.7554/eLife.59754

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020, Savier et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

PMC Copyright notice

Figure 3. — (A) Images of two experimental injections showing the collicular terminations zones (triangles and square, top-view, upper panels) after focal retinal injections (arrows, flat-mount, lower panel) in Isl2^Epha3/+ animals. (B) Cartesian representation of the injections (triangles and square) in (A) superimposed with the simulated RC map (black dots, n = 100) in Isl2^Epha3/+. Map profile is calculated by LOESS smoothing (black and gray lines). (C) Images of two experimental injections showing the collicular termination zones (sagittal view, upper panels) after focal cortical V1 injection (top-view, lower panels). Arrows and arrowheads indicate the site of the termination zones. Lower left panel shows CO staining (dark gray) delineating V1. (D) Cartesian representation of the experimental (red dots/lines, n = 15 animals) and simulated (black dots, n = 100) CC maps calculated by LOESS smoothing (black, red and gray lines). Arrows and arrowhead represent the two injections shown in (C). Two-samples Kolmogorov-Smirnov test, D-stat = 0.273 < D-crit.=0.282, p=0.06, simulated and experimentally measured CC maps are not significantly different. (E) Images of two experimental injections showing the collicular terminations zones (triangles and squares, top-view, upper panels) after focal retinal injection (arrows, flat-mount, lower panel) in Isl2^Epha3/Epha3 animals. (F) Cartesian representation of the injections (triangles and squares) in (E) superimposed with the simulated RC map (black dots, n = 100) in Isl2^Epha3/Epha3. Map profile is calculated by LOESS smoothing (black and gray lines). (G) Images of two experimental injections showing the collicular duplicated termination zones (arrows and arrowheads, sagittal view, upper panels) after focal cortical V1 injection (top-view, lower panels). (H) Cartesian representation of the experimental (red dots/lines, n = 7 animals) and simulated (black dots, n = 100) CC maps calculated by LOESS smoothing (black, red and gray lines). Arrows and arrowheads represent the two examples in (G). Two-samples Kolmogorov-Smirnov test, D-stat = 0.190 < D-crit.=0.371, p=0.72, simulated and experimentally measured CC maps are not significantly different. Scale bars: 400 μm (A upper, C, E upper, G), 1 mm (A, E lower). Abbreviations: N, nasal; T, temporal; R, rostral; C, caudal; M, medial; L, lateral.

Figure 3—source data 1. Data for Figure 3B: Cartesian values of experimentally measured collicular termination zones and corresponding retinal injections in Isl2^Epha3/+ animals.
Data for Figure 3D: Cartesian values of experimentally measured collicular termination zones and corresponding cortical V1 injections in Isl2^Epha3/+ animals. Data for Figure 3F: Cartesian values of experimentally measured collicular termination zones and corresponding retinal injections in Isl2^Epha3/Epha3 animals. Data for Figure 3B: Cartesian values of experimentally measured collicular termination zones and corresponding cortical V1 injections in Isl2^Epha3/Epha3 animals.

elife-59754-fig3-data1.xlsx^{(12.5KB, xlsx)}