Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus. musculus) | Isl2tm1(Epha3)Grl | Lemke Lab (Salk Institute) | MGI:3056440 RRID:MGI:3057124 | |
| Commercial assay, kit | Neural tissue dissociation Kit – Postnatal neurons | Miltenyi | Cat # 130-094-802 | |
| Commercial assay, kit | RNeasy Mini kit | Qiagen | Cat # 74104 | |
| Commercial assay, kit | QuantiTect SYBR Green RT-PCR Kit | Qiagen | Cat # 204243 | |
| Antibody | Goat anti-rabbit IgG (H+L), polyclonal | Jackson Immunoresearch | Cat # 111-005-003 RRID:AB_2337913 | 1:3000 |
| Antibody | Goat anti-mouse IgM (H+L) | Biotrend | Cat # 610–1107 | 1:3000 |
| Antibody | Mouse anti-CD90, monoclonal | Biorad | Cat # MCA02R F7D5 IgM RRID:AB_323481 | 1:3000 |
| Antibody | Rabbit anti-rat macrophage, polyclonal | Life Science | Cat # AIA51240 | 1:3000 |
| Chemical compound, drug | Neurobasal medium | Gibco/Thermofisher | Cat # 21103049 | |
| Chemical compound, drug | B27 | Thermofisher | Cat # 17504044 | |
| Chemical compound, drug | BDNF | PeproTech | Cat # 450–02 | 25 ng/ml |
| Chemical compound, drug | CNTF | PeproTech | Cat # 450–13 | 10 ng/ml |
| Chemical compound, drug | Forskolin | Sigma | Cat # F3917 | 10 mM |
| Chemical compound, drug | Glutamine | Thermofisher | Cat # 25030149 | 2 mM |
| Chemical compound, drug | N-acetyl-l-cysteine | Sigma | Cat # A09165 | 60 mg/ml |
| Chemical compound, drug | Penicilin/streptomycin | Gibco | Cat # 15070–022 | 100 units/ml |
| Chemical compound, drug | Sodium pyruvate | Thermofisher | Cat # 11360070 | 1 mM |
| Chemical compound, drug | DiI (1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate) | Thermofisher | Cat # D282 | |
| Chemical compound, drug | DiD (1,1’–dioctadecyl-3,3,3’,3’- tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate) | Thermofisher | Cat # D7757 | |
| Software, algorithm | R Project for Statistical Computing | www.r-project.org | RRID:SCR_001905 | |
| Software, algorithm | ImageJ | https://imagej.nih.gov/ij | RRID:SCR_003070 | |
| Software, algorithm | MATLAB | RRID:SCR_001622 | ||
| Software, algorithm | 3-step map alignment | Reber Lab (Krembil) | https://github.com/michaelreber/3-step-Map-Aligment-Model/blob/master/threestepsMapAlignment.m | |
| Software, algorithm | LOESS smoothing | Reber Lab (Krembil) | https://github.com/michaelreber/Leave-one-out-LOESS/blob/master/wtloess.R |