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. 2020 Jul 7;25(9):674–685. doi: 10.1007/s10495-020-01620-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Costunolide-induced eryptosis and cell shrinkage in mature human erythrocytes. Original histograms of annexin V-binding (a), concentration-dependent increase of annexin V-binding cells (b), concentration-dependent effect on hemolysis (c), original histograms of forward scatter (FCS) (d), and concentration-dependent decrease of forward scatter (FCS) (e) after treatment of human erythrocytes for 24 h with costunolide are shown. Number of independent experiments: n = 3. Differences of the means were considered to be statistically significant when the calculated p value was less than 0.05 (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)