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. 2020 Sep 30;3:541. doi: 10.1038/s42003-020-01243-2

Fig. 7. Pancreas-wide GLUT2 downregulation following STZ-induced hyperglycemia is partially recovered by islet transplantation into the ACE.

Fig. 7

a Blood glucose levels were measured during a period of 28 days after SHD-STZ (150 mg/kg) or vehicle injection. Four days after STZ administration, hyperglycemic mice were either syngeneically transplanted with ~100–150 islets into the ACE to restore normoglycemia (SHD-STZ + Tx), or left untransplanted (SHD-STZ). n (animals) = 13 for control, n = 15 for SHD-STZ, and n = 8 for SHD-STZ + Tx. Pancreata were harvested 28 days post-STZ administration for ex vivo OPT imaging or islet isolation. Gray shading represents normoglycemic levels, as defined by blood glucose concentration ≤ 12 mmol/L. b Analysis of GLUT2 expression in pancreatic islets reveals a partial recovery of GLUT2 expression in transplanted mice. Representative OPT renderings show pancreatic splenic lobes labeled for insulin (red), glucose transporter GLUT2 (blue), and overlay images (co-staining indicated by yellow color) in vehicle treated controls (n = 7), SHD-STZ (n = 8), and SHD-STZ + Tx (n = 4). c Quantitative assessment of the number of co-localizing voxels based on insulin and GLUT2 in control, SHD-STZ and SHD-STZ + Tx pancreata respectively. d Whole islet insulin content from isolated islets, normalized to DNA content. e Relative expression of genes associated to β-cell function, maturity and dedifferentiation determined by qRT-PCR on cDNA from isolated islets. d, e n = 5, 6, and 4 mice for vehicle control, SHD-STZ, SHD-STZ + Tx, respectively. Error bars represent SEM (a, c) or SD (d, e). *, **, ***, and ****represent P ≤ 0.05, ≤0.01, ≤0.001, and ≤0.0001 respectively.