Table 5.

Nucleotide sequences of the real-time RT-PCR primers used in this study.

Primer name	Abbreviation	Direction	Sequence (5′–3′)	Amplicon length
Phenylalanine ammonia-lyase	PAL	Forward	ACGGGTTGCCATCTAATCTGACA	92
		Reverse	CGAGCAATAAGAAGCCATCGCAAT
Cinnamic acid 4-hydroxylase	C4H	Forward	CCCAGTTTTTGGAAATTGGCTTCA	104
		Reverse	GCCCCATTCTAAGCAAGAGAACATC
Hydroxycinnamoyl Co A shikimate hydroxycinnamoyl transferase	HCT	Forward	TCTCCAACCCCTTTTAACGAACC	103
		Reverse	CAACTTGTCCTTCTACCACAGGGAA
p-coumarate 3-hydroxylase	C3H	Forward	TTGGTGGCTACGACATTCCTAAGG	100
		Reverse	GGTCTGAACTCCAATGGGTTATTCC
Hydroxycinnamoyl Co A quinate hydroxycinnamoyl transferase	HQT	Forward	CCCAATGGCTGGAAGATTAGCTA	99
		Reverse	CATGAATCACTTTCAGCCTCAACAA
Chalcone synthase	CHS	Forward	CACCGTGGAGGAGTATCGTAAGGC	93
		Reverse	TGATCAACACAGTTGGAAGGCG
Chalcone isomerase	CHI	Forward	GGCAGGCCATTGAAAAGTTCC	103
		Reverse	CTAATCGTCAATGATCCAAGCGG
Flavanone 3-hydroxylase	F3H	Forward	CCAAGGCATGTGTGGATATGGACC	103
		Reverse	CCTGGATCAGTATGTCGTTCAGCC
Flavonol synthase	FLS	Forward	CCTCCTTCCTACAGGGAAGCAAA	91
		Reverse	CAAGCCCAAGTGACAAGCTCCTAA
Dihydroflavonol 4-reductase	DFR	Forward	TCACAGGAGCAGCTGGATTTATCG	91
		Reverse	TCAGGATCACGAACAGTAGCATGG
Flavonoid 3′ hydroxylase	F3′H	Forward	TGGGTATACCCAAACTCATTCCG	96
		Reverse	AAAAGCCCAAAGTTGATGTGAAAGG
Anthocyanin 1 transcription factor	AN1	Forward	CCTCAACCTCAGAAATTCAGAAGC	102
		Reverse	TCGTTGTTGTTGTCGTTCGATGC
Anthocyanin 2 transcription factor	AN2	Forward	ACAAGATGCCACTTTCCTTCACC	101
		Reverse	TGTGCATCGTTGGGAGTTAGG
Elongation factor 1-alpha	EF1-α	Forward	ATTGGAAACGGATATGCTCCA	101
		Reverse	TCCTTACCTGAACGCCTGTCA
Alfalfa mosaic virus-coat protein	AMV-CP	Forward	CCATCATGAGTTCTTCACAAAAG	151
		Reverse	TCGTCACGTCATCAGTGAGAC