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. 2020 Sep 30;10:16182. doi: 10.1038/s41598-020-73411-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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EV characterization. (a, b) EV concentration (particles/mL) and nanometer diameter (nm) were assessed by NTA in patients with a diagnosis of STEMI (n = 7) before PCI and after 24 h from reperfusion, as compared to controls (Ctrl; n = 9). (c) Percentage value of median fluorescence intensity (% MFI) for CD9, CD63, and CD81 assessed by flow cytometry. (d) Western blot analysis for EV specific markers (TSG101 and CD81) in representative patients (n = 4). Level of expression are reported as arbitrary unit (a.u.) in patients with STEMI before and 24 h after reperfusion, as compared to controls. Uncropped representative membranes are shown in Fig. S1c,d. Data and statistical analysis are reported in Table S1. *p < 0.05; **p < 0.01.