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. 2020 Sep 17;11:581508. doi: 10.3389/fmicb.2020.581508

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Copyright © 2020 Rai, Fisher, Duckworth and Baars.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Analysis of siderophore standards (6.25 μM) in water immediately after addition of (1) 100 μM FeCl₃; (2) 100 μM FeCl₃, 10 mM CaCl₂, 2.5 mM ascorbate; (3) 100 μM FeCl₃, 2.5 mM ascorbate, (4) 100 μM FeCl₃, 1mM EDTA. (B) LC-MS peak of a rhizoferrin standard (3.1 μM) in water and comparison to the same standard after addition of 100 μM FeCl₃. (C) LC-MS signals of 3.1 μM rhizoferrin/100 μM FeCl₃ after addition of Ca²⁺ (10 mM) and ascorbate (2.5 mM) and comparison to ascorbate only or Ca²⁺ only.