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. 2020 Sep 17;11:2196. doi: 10.3389/fimmu.2020.02196

Figure 3.

Figure 3

Scaffolding LSD1 inhibitor-induced oxidative stress in NK cells is dose dependent and can be rescued with glutathione supplementation, but metabolism defects cannot. (A) MitoSOX dose response of SP-2509 and SP-2577 in live NK cells at indicated time points and rescued using 2.5 mM glutathione ethyl ester (GSHee). (B) Glutathione dose response of SP-2509 and SP-2577 in live NK cells measured using mBCL and rescued using 2.5 mM GSHee. (C) MitoTracker dose response of SP-2509 and SP-2577 in live NK cells and attempted rescued using 2.5 mM GSHee. (D) OXPHOS of NK cells treated with scaffolding LSD1 inhibitors for 48 h and attempted rescue with cell-wide antioxidants (GSHee and Trolox) and mitochondrial-targeted antioxidants [mitoquinol (MQ) and SKQ1] measured using XF Mito Stress Test. (E) Basal glycolysis of NK cells treated with scaffolding LSD1 inhibitors for 48 h using the same method and measured using XF Mito Stress test. *q < 0.01. All conditions are compared to DMSO control via t-test with FDR correction. Marked Seahorse data points indicate all treatment conditions are significant vs. DMSO control. At least three independent experiments are displayed (± SEM), sourced from two unique NK cell donors.