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. 2020 Sep 17;11:2196. doi: 10.3389/fimmu.2020.02196

Figure 4.

Figure 4

NK cell ligand expression and cytotoxicity are impaired by scaffolding LSD1 inhibitors, but viability and cytotoxicity can be rescued with glutathione supplementation. (A) NK cells treated for 48 h with indicated LSD1 inhibitors display reduced activating ligand expression. (B) Viability dose response of SP-2509 and SP-2577 in NK cells treated with and without 2.5 mM GSHee supplementation. (C) Flow cytometry plots of NK cells treated with SP-2509 at indicated doses for 48 h, with and without 2.5 mM GSHee supplementation. (D) Quantification of flow cytometry gates from panel C across three unique NK donors. (E) NK cell cytotoxicity against K562 target cells is reduced by 48 h pre-treatment with indicated LSD1 inhibitors. (F) NK cell cytotoxicity against MOLM13 target cells after 48 h pre-treatment with SP-2509. (G) NK cell cytotoxicity against K562 target cells after 48 h pre-treatment with SP-2509, with and without 2.5 mM GSHee supplementation. (H) Working model of scaffolding LSD1 inhibitor effects on NK cell metabolism, redox state, and function. *q < 0.01. All conditions are compared to DMSO control via t-test with FDR correction. At least three independent experiments are displayed (± SEM), sourced from three unique NK cell donors.