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. 2020 Sep 30;11:4909. doi: 10.1038/s41467-020-18626-y

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Flow cytometric analysis of M2-like macrophages (CD206⁺) and M1-like macrophages (CD80⁺) in tumor gating on F4/80⁺CD11b⁺CD45⁺ cells. b Flow cytometric analysis of CD8⁺ and CD4⁺ T cells in tumor gating on CD45⁺ cells. c Immunohistochemistry images of tumors showing CD8⁺ T cells in different treatment groups. Scale bar, 50 µm. d Multiplex immunohistochemistry images of tumors showing CD8⁺ T cell and F4/80⁺ macrophage infiltration in different treatment groups. Scale bar, 100 µm. e Cytokine levels in tumors from mice isolated 5 days after different treatments. All data are presented as mean ± S.D. (n = 4). Statistical significance was calculated via ordinary one-way ANOVA with a Tukey’s test. *P < 0.05; **P < 0.01; ***P < 0.001.