Figure 3. HOTAIR was a sponge of miR-874-5p. (A) LncBase Predicted v.2 predicted that HOTAIR and miR-874-5p had targeted binding sites. (B) The luciferase activity was detected using dual-luciferase reporter assay in SK-N-SH cells co-transfected with HOTAIR WT or HOTAIR MUT and miR-874-5p mimics or miR-NC. (C) RIP analysis was performed to verify the relationship between HOTAIR and miR-874-5p. (D) The inhibition efficiency of anti-miR-874-5p was determined by qRT-PCR. (E) The level of miR-874-5p was examined by qRT-PCR in SK-N-SH cells transfected with si-NC or si-HOTAIR. (F) The expression of miR-874-5p was measured in SK-N-SH cells stimulated with different doses of MPP+ (0 mM, 0.25 mM, 0.5 mM and 1 mM) for 24 h. (G) The level of miR-874-5p was detected in SK-N-SH cells incubated with 1 mM MPP+ for 0 h, 12 h, 24 h or 48 h. *P < 0.05.
